Vol. 7, Issue 3, Part C (2024)

Materials and Methods: The present study was carried out in Central Haematology Lab, Department of Pathology, B.J. Medical college, Ahmedabad from period of August 2023 to November 2023 with 581 cases evaluated with an aim to identify various hemoglobinopathies seen in Indian population by high-performance liquid chromatography.

Results: A comprehensive analysis of 581 cases revealed that 321 (55.2%) had abnormal hemoglobinopathies, including Beta thalassemia trait (75.1%), Sickle cell trait (9.9%), Double heterozygous for thalassemia and sickle cell (5.3%), Sickle cell disease (2.5%), Hb D (2.2%), Iron Deficiency Anemia (IDA) (1.9%), Hb E (1.6%), Thalassemia major (0.6%), Beta delta homozygous HPFH(Hereditary Persistence of Fetal Hemoglobin) (0.6%), and Thalassemia intermedia (0.3%), while 260 cases (44.8%) had normal chromatograms, with 3 samples requiring repeat testing, 7 cases reserved for further opinion, and the remaining cases showing no abnormalities.

Conclusion: This study demonstrates the effectiveness of HPLC in diagnosing hemoglobinopathies in a resource-constrained setting. The high prevalence of Beta thalassemia trait and disease underscores the need for widespread adoption of HPLC technology in regions like India, where early detection and management can significantly impact patient outcomes. Our findings highlight the importance of HPLC as a rapid, accurate, and essential tool in the diagnosis and management of hemoglobin disorders.