Study of CD73 Expression on B Lymphocytes in patients with chronic lymphocytic leukemia
Hany Mostafa Kamel El Zohery, Amr Mohamed Gawaly, Wesam Salah Mohamed and Aisha Abdellatif El Said
Background: A subgroup of T and B cells express the extracellular enzyme cluster of differentiation (CD73), which hydrolyzes adenosine monophosphate to adenosine. This work aimed to evaluate CD73 expression and its correlation with other disease characteristics in leukemia of chronic lymphocytic type (CLL) patients.
Methods: This cross-sectional research was done on 30 CLL cases diagnosed by laboratory investigations or presented with fatigue and fever with or without lymphadenopathy and organomegaly, and a healthy control group of 30 age- and sex-matched individuals. All patients had subjected to estimation of β2 microglobulin, flowcytometric detection of CD73 and CD19 in peripheral blood.
Results: In neither group did CD19/CD73 expression and haemoglobin significantly correlate. microglobulin in control group. While, the CD19/CD73 expression had positive significant correlation with B2 macroglobulin in CLL group. In the CLL group, haemoglobin level, platelet count, and CD19/CD73 expression were all noticeably reduced. B2 microglobulin, however, was noticeably greater in the CLL group. CD19/CD73 expression can diagnose CLL with high accuracy (100%) at cut-off 16.8, sensitivity, specificity, PPV and NPV were 100%.
Conclusions: When compared to controls, most CLL patients have less expression of CD19CD73, making it useful in standard diagnostic immunophenotyping workup. At the cut-off point of 16.8, CD19/CD73 expression has a high sensitivity (100%) for the diagnosis of CLL.
