Vol. 6, Issue 2, Part A (2023)

Methods: This cross-sectional research was done on 30 CLL cases diagnosed by laboratory investigations or presented with fatigue and fever with or without lymphadenopathy and organomegaly, and a healthy control group of 30 age- and sex-matched individuals. All patients had subjected to estimation of β2 microglobulin, flowcytometric detection of CD73 and CD19 in peripheral blood.

Results: In neither group did CD19/CD73 expression and haemoglobin significantly correlate. microglobulin in control group. While, the CD19/CD73 expression had positive significant correlation with B2 macroglobulin in CLL group. In the CLL group, haemoglobin level, platelet count, and CD19/CD73 expression were all noticeably reduced. B2 microglobulin, however, was noticeably greater in the CLL group. CD19/CD73 expression can diagnose CLL with high accuracy (100%) at cut-off 16.8, sensitivity, specificity, PPV and NPV were 100%.

Conclusions: When compared to controls, most CLL patients have less expression of CD19CD73, making it useful in standard diagnostic immunophenotyping workup. At the cut-off point of 16.8, CD19/CD73 expression has a high sensitivity (100%) for the diagnosis of CLL.