Vol. 4 Issue 4 Part B

Methods: The present Prospective study was conducted in the Department of Surgery and Medicine and in collaboration with pathology department of SGT Medical College Hospital & Research Institute Budhera, Gurugram, Haryana, India, from June 2020 to May 2021. 500 patients’ comprised 350 adults and 150 children for Hb estimation. Hb estimation was done simultaneously both by Mission Ultra Hb Meter and Haematology Analyser i3.

Result: A total of 500 samples were processed for hemoglobin estimation. Out of these 225 were males and 275 females. Mean haemoglobin concentration on Mission Ultra Hb Meter for adults and children were 10.88+/- 2.36 and 10.22+/-2.51 respectively. Mean hemoglobin concentration by Hematology Analyzer i3 for adults and children were 10.98+/-2.15 and 10.52+/-2.52 respectively. This shows mean by Hematology Analyzer i3 is greater by 2.17% for adults and 3.06% for children. It takes less than 1 minute by Mission Ultra Hb Meter while 15- 20 minutes by HematologyAnalyzeri3. Mission Ultra Hb Meter requires almost negligible maintenance while automated analyzer regular maintenance. The mean (SD) hemoglobin level was 10.55 ± 0.52and 10.75 ± 0.63g/dl using Mission Ultra Hb Meter vs HematologyAnalyzeri3. There was a positive correlation (r = 0.51, P < 0.001) between hemoglobin levels by using Mission Ultra Hb Meter vs HematologyAnalyzer i3. The Bias of agreement was 0.4g/dl resulting to 95% correlation. We also observed that out of 500 patients tested 53 were true anemic when tested from HematologyAnalyzer i3. In comparison Mission Ultra Hb Meter provided a Sensitivity and Specificity of 92% and 96% in detection of anemia.

Conclusion: Hemoglobin concentration assessment by Mission Ultra Hb Meter using venous blood samples has shown acceptable agreement with HematologyAnalyzeri3.