Evaluation of sperm DNA abnormality index as a indicator of male infertility

Dr. Pushpalatha D; Dr. Shilender Tamaskar; Dr. Sundaram

doi:10.33545/pathol.2021.v4.i1b.334

Evaluation of sperm DNA abnormality index as a indicator of male infertility

Dr. Pushpalatha D, Dr. Shilender Tamaskar and Dr. Sundaram

DOI: https://doi.org/10.33545/pathol.2021.v4.i1b.334

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ABSTRACT

Aim and objective: To assess the prevalence of sperm DNA integrity in both normal and abnormal subjects by a simple and cost effective cyto-chemical method using toluidine blue stain, and to analyse the value of sperm DNA integrity as an efficient tool in male infertility.

Material and methods: Samples were collected from men visiting for evaluation of infertility. Routine semen parameter was assessed as per WHO manual 5^thEdition and abnormal sperm DNA integrity index was calculated using toluidine blue method. Samples with abnormal semen parameter was taken as study group, sample from healthy proven voluntary donors were taken as control group. Abnormal sperm DNA integrity index was then correlated with the study groups.

Results: Abnormal sperm DNA integrity index also correlated well with the fertility potential.

International Journal of Clinical and Diagnostic Pathology

International Journal of Clinical and Diagnostic Pathology

Evaluation of sperm DNA abnormality index as a indicator of male infertility